Jones SL, Harris K, and Geyer CB (2019) A new translation and reader's guide to Victor von Ebner's classical description of spermatogenesis. Mol Reprod Dev Epub ahead of print.
Hale BJ, Fernandez RF, Kim SQ, Diaz VD, Jackson SN, Liu L, Brenna JT, Hermann BP, Geyer CB*, Ellis JM* (2019) Acyl-CoA Synthetase 6 enriches seminiferous tubules with the omega-3 fatty acid DHA and is required for male fertility in the mouse. J Biol Chem Epub ahead of print (http://www.jbc.org/content/early/2019/08/09/jbc.RA119.009972.long)
*Co-corresponding senior authors.
Velte EK, Niedenberger BA, Serra ND, Singh A, Roa-DeLaCruz L, Hermann BP, and Geyer CB (2019) Differential RA responsiveness directs formation of functionally-distinct spermatogonial populations at the initiation of spermatogenesis in the mouse. Development 146(12). (http://dev.biologists.org/content/early/2019/04/19/dev.173088.long)
This report is the first to show molecular heterogeneity of quiescent prospermatogonia/gonocytes, which are the precursors to spermatogonia. This indicates that establishment of the fates (stem vs progenitor vs differentiating) of the nascent spermatogonial populations are already in-progress in this stage, which was long-thought to contain homogeneous cells with equipotent fates.
Serra ND, Velte EK, Niedenberger BA, Kirsanov O, and Geyer CB (2019) The mTORC1 component RPTOR is required for maintenance of the foundational spermatogonial stem cell pool in mice. Biol Reprod 100(2): 429-439. (https://academic.oup.com/biolreprod/article/100/2/429/5094553)
Hermann BP, Cheng K, Singh A, Roa-De La Cruz L, Mutoji KN, Chen IC, Gildersleeve H, Lehle JD, Mayo M, Weternstroer B, Law NC, Oatley MJ, Velte EK, Niedenberger BA, Fritze D, Silber S, Geyer CB, Oatley JM, and McCarrey JR. The mammalian spermatogenesis single-cell transcriptome, from spermatogonial stem cells to spermatids. Cell Rep 25(6): 1650-1667. (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6384825/)
Niedenberger BA, Cook K, Baena V, Serra ND, Velte EK, Agno JE, Litwa KA, Terasaki M, Hermann BP, Matzuk MM, Geyer CB. (2018) Dynamic cytoplasmic projections connect mammalian spermatogonia in vivo. Development, 145(15). (http://dev.biologists.org/content/early/2018/07/04/dev.161323.long)
Sertoli E (2018) The structure of seminiferous tubules and the development of [spermatids] in rats*. Biol Reprod, epub ahead of print. (https://academic.oup.com/biolreprod/advance-article/doi/10.1093/biolre/ioy134/5046415)
*The Geyer lab commissioned the English translation and recreation of the figures from this largely unknown work published in 1878 by the famous Italian histologist Enrico Sertoli. In addition, Chris spent a considerable amount of time to modernize terminology and attempt to frame the results of this exciting chapter in the context of what we now know about mammalian spermatogenesis.
Geyer CB (2018) A historical perspective on some 'new' discoveries on spermatogenesis from the laboratory of Enrico Sertoli in 1878**. Biol Reprod 99(3): 479-481. (https://academic.oup.com/biolreprod/article/99/3/479/4494360)
**This is an accompaniment to an English translation we commissioned of Sertoli's chapter originally published in 1878 entitled "The structure of seminiferous tubules and the development of spermatids in rats". Chris described the methods used in the translation and highlights the discoveries that Sertoli made for which he unfortunately never received due credit.
Niedenberger BA and Geyer CB (2018) Advanced immunostaining approaches to study early male germ cell development. Stem Cell Res 27: 162-168. (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5894494/)
Serra ND, Velte EK, Niedenberger BA, Kirsanov O, and Geyer CB (2017) Cell-autonomous requirement for mammalian target of rapamycin (Mtor) in spermatogonial proliferation and differentiation in the mouse. Biol Reprod 96(4): 816-828. (https://academic.oup.com/biolreprod/article-lookup/doi/10.1093/biolre/iox022)
Mutoji K, Singh A, Nguyen T, Gildersleeve H, Kaucher AV, Oatley MJ, Oatley JM, Velte E, Geyer CB, Cheng K, McCarrey JR, and Hermann BP (2016) TSPAN8 expression distinguishes spermatogonial stem cells in the prepubertal mouse testis. Biol Reprod 95(6): 117. (https://academic.oup.com/biolreprod/article-lookup/doi/10.1095/biolreprod.116.144220)
Busada JT, Velte EK, Serra N, Cook K, Niedenberger BA, Willis WD, Goulding EH, Eddy EM, and Geyer CB (2016) Rhox13 is required for a quantitatively normal first wave of spermatogenesis in mice. Reproduction 152(5): 379-388. (http://www.reproduction-online.org/content/152/5/379.long)
Busada JT and Geyer CB (2015) The role of retinoic acid (RA) in spermatogonial differentiation. Biol Reprod 94(1): 10. (https://academic.oup.com/biolreprod/article-lookup/doi/10.1095/biolreprod.115.135145)
Busada JT, Niedenberger BA, Velte EK, Keiper BD, and Geyer CB (2015) Mammalian target of rapamycin complex 1 (mTORC1) is required for mouse spermatogonial differentiation in vivo. Dev Biol 407(1): 90-102. (http://www.sciencedirect.com/science/article/pii/S0012160615301020?via%3Dihub)
Niedenberger BA, Busada JT, and Geyer CB (2015) Marker expression reveals heterogeneity of spermatogonia in the neonatal testis. Reproduction 149(4): 329-338. (http://www.reproduction-online.org/content/149/4/329.long)
Hermann BP, Mutoji K, Velte EK, Daijin K, Oatley JM, Geyer CB, and McCarrey JR (2015) Transcriptional and translational heterogeneity among neonatal mouse spermatogonia. Biol Reprod 92(2): 54. (https://academic.oup.com/biolreprod/article-lookup/doi/10.1095/biolreprod.114.125757)
Busada JT, Chappell VA, Niedenberger BA, Kaye EP, Keiper BD, Hogarth CA, and Geyer CB (2015) Retinoic acid regulates Kit translation during spermatogonial differentiation in the mouse. Dev Biol 397(1): 140-9. (http://www.sciencedirect.com/science/article/pii/S0012160614005612)
Niedenberger BA, Chappell VA, Otey CA, and Geyer CB (2014) Actin dynamics regulate subcellular localization of PALLD in mouse Sertoli cells. Reproduction 148(4): 333-41. (http://www.reproduction-online.org/content/148/4/333.long)
Busada JT, Kaye EP, Renegar RH, and Geyer CB (2014) Retinoic acid induces multiple hallmarks of the prospermatogonia-to-spermatogonia transition in the neonatal mouse. Biol Reprod 90(3): 64. (https://academic.oup.com/biolreprod/article-lookup/doi/10.1095/biolreprod.113.114645)
Chappell VA, Busada JT, Keiper BD, and Geyer CB (2013) Translational activation of developmental mRNAs during neonatal testis development. Biol Reprod 89(3): 61, 1-10. (https://academic.oup.com/biolreprod/article-lookup/doi/10.1095/biolreprod.113.109819)
Niedenberger BA, Chappell VK, Kaye EP, Renegar RH, and Geyer CB (2013) Nuclear localization of the actin binding protein Palladin in Sertoli cells. Mol Reprod Dev 80(5): 403-413. (http://onlinelibrary.wiley.com/doi/10.1002/mrd.22174/abstract;jsessionid=9D8479614768EFBD501070110B30335F.f04t01)
Overcash RF, Chappell VA, Green T, Geyer CB, Asch AS, and Ruiz-Echevarria, MJ (2013) Androgen signaling promotes translation of TMEFF2 in prostate cancer cells via phosphorylation of the a subunit of the translation initiation factor 2 (eIF2a). PLoS One 8(2): e55257. (http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0055257)
Geyer CB, Saba R, Kato Y, Anderson AJ, Chappell VK, Saga Y, and Eddy EM (2010) Rhox13 is translated in premeiotic germ cells in male and female mice and translation is regulated by NANOS2 in the male. Biol Reprod 86(4): 1-9. (https://academic.oup.com/biolreprod/article-lookup/doi/10.1095/biolreprod.111.094938)
Danshina PV, Geyer CB, Dai Q, Goulding EH, Willis WD, Kitto GB, McCarrey JR, Eddy EM, O’Brien DA (2009) Phosphoglycerate kinase 2 (PGK2) is essential for sperm function and male fertility in mice. Biol Reprod 82(1): 136-145.
Geyer CB, Inselman AI, Sunman J, Bornstein S, Handel MA, Eddy EM (2009) A missense mutation in the Capza3 gene and disruption of F-actin organization in spermatids of repro32 infertile male mice. Dev Biol 330(1): 142-152.
Geyer CB and Eddy EM (2008) Identification and characterization of Rhox13, a novel X-linked mouse homeobox gene. Gene 423(2): 194-200.
Yoshioka H, Geyer CB, Hornecker JL, Patel KT, McCarrey JR (2007) In vivo analysis of developmentally and evolutionarily dynamic protein-DNA interactions regulating transcription of the Pgk2 gene during mammalian spermatogenesis. Mol Cell Biol 27(22): 7871-7885.
McCarrey JR, Geyer CB, Yoshioka H (2005) Epigenetic regulation of testis-specific gene expression. Ann NY Acad Sci 1061: 226-242.
Geyer CB, Kiefer CM, Yang T, McCarrey JR (2004) Ontogeny of a demethylation domain and its relationship to activation of tissue-specific transcription. Biol Reprod 71: 837-844.
Keiser JT, Jobst PM, Garst AS, Boone JT, Geyer CB, Phelps C, Ayares DL, Page RL (2001) Preimplantation screening for transgenesis using an embryonic specific promoter and green fluorescent protein. Cloning 3(1): 23-30.